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Persistent URL http://purl.org/net/epubs/work/50384
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Record Id 50384
Title Molecular replacement using ab initio polyalanine models generated with ROSETTA
Abstract The success of the molecular-replacement method for solving protein structures from experimental diffraction data depends on the availability of a suitable search model. Typically, this is derived from a previously solved structure, sometimes by homology modelling. Very recently, Baker, Read and coworkers have demonstrated a successful molecular-replacement case based on an ab initio model generated by ROSETTA [Qian et al. (2007), Nature (London), 450, 259?264]. In this contribution, a number of additional test cases in which ab initio models generated using modest computational resources give correct molecular-replacement solutions are reported. Unsuccessful cases are also reported for comparison and the factors influencing the success of this route to structure solution are discussed.
Organisation CSE , CCP4 , STFC
Keywords Biology , molecular replacement
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Language English (EN)
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Journal Article Acta Crystallogr D D64 (2008): 1288-1291. doi:10.1107/S0907444908033192 2008