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Full Record Details
Persistent URL
http://purl.org/net/epubs/work/50384
Record Status
Checked
Record Id
50384
Title
Molecular replacement using ab initio polyalanine models generated with ROSETTA
Contributors
DJ Rigden (Liverpool U.)
,
RM Keegan (STFC Daresbury Lab.)
,
MD Winn (STFC Daresbury Lab.)
Abstract
The success of the molecular-replacement method for solving protein structures from experimental diffraction data depends on the availability of a suitable search model. Typically, this is derived from a previously solved structure, sometimes by homology modelling. Very recently, Baker, Read and coworkers have demonstrated a successful molecular-replacement case based on an ab initio model generated by ROSETTA [Qian et al. (2007), Nature (London), 450, 259?264]. In this contribution, a number of additional test cases in which ab initio models generated using modest computational resources give correct molecular-replacement solutions are reported. Unsuccessful cases are also reported for comparison and the factors influencing the success of this route to structure solution are discussed.
Organisation
CSE
,
CCP4
,
STFC
Keywords
Biology
,
molecular replacement
Funding Information
Related Research Object(s):
Licence Information:
Language
English (EN)
Type
Details
URI(s)
Local file(s)
Year
Journal Article
Acta Crystallogr D
D64 (2008): 1288-1291.
doi:10.1107/S0907444908033192
2008
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